A nano particle vector comprised of poly lactic-co-glycolic acid and monophosphoryl lipid A and recombinant Mycobacterium avium subsp paratuberculosis peptides stimulate a pro-immune profile in bovine macrophages

Authors

• C.D. Souza, Washington State University, PullmanFollow
• John Bannantine, USDA ARS National Animal Disease CenterFollow
• W.C. Brown, Washington State University, Pullman
• M.G. Norton, Washington State University, Pullman
• W.C. Davis, Washington State University, Pullman
• J.K. Hwang, Washington State University, Pullman
• P. Ziaei, Washington State University, Pullman
• Gaber S. Abdellrazeq, Washington State University, Pullman & Alexandria University
• M.V. Eren, Washington State University, Pullman
• J.R. Deringer, Washington State University, Pullman
• E. Laws, Washington State University, Pullman
• M.C.D. Cardieri, Washington State University, Pullman

ORCID IDs

http://orcid.org/0000-0002-8677-911X

Document Type

Article

Date of this Version

2017

Citation

Journal of Applied Microbiology 123, 54--65

doi:10.1111/jam.13491

Comments

2017 The Society for Applied Microbiology

Abstract

Aims: We evaluated the potential of a nanoparticle (NP) delivery system to improve methods of delivery of candidate peptide-based vaccines for Paratuberculosis in cattle.

Methods and Results: Peptides derived from Mycobacterium avium subsp. paratuberculosis (Map), and the pro-inflammatory monophosphoryl lipid A (MPLA) were incorporated in polymeric NPs based on poly (D,L-lactide-co- glycolide) (PLGA). The PLGA/MPLA NPs carriers were incubated with macrophages to examine their effects on survival and function. PLGA/MPLA NPs, with and without Map antigens, are efficiently phagocytized by macrophages with no evidence of toxicity. PLGA/MPLA NP formulations did not alter the level of expression of MHC I or II molecules. Expression of TNFa and IL12p40 was increased in Map-loaded NPs. T-cell proliferation studies using a model peptide from Anaplasma marginale demonstrated that a CD4 T- cell recall response could be elicited with macrophages pulsed with the peptide encapsulated in the PLGA/MPLA NP.

Conclusions: These findings indicate PLGA/MPLA NPs can be used as a vehicle for delivery and testing of candidate peptide-based vaccines.

Significance and Impact of the Study: These results will assist on more in depth studies on PLGA NP delivery systems that may lead to the development of a peptide-based vaccine for cattle.