Phenotype and Function of CD209+ Bovine Blood Dendritic Cells, Monocyte-Derived- Dendritic Cells and Monocyte-Derived Macrophages

Authors

Kun Taek Park, Washington State University, Pullman & Seoul National University
Mahmoud M. Elnaggar, Washington State University, Pullman &Alexandria University, Egypt
Gaber S. Abdellrazeq, Washington State University, Pullman & Alexandria University
J. P. Bannantine, USDA-ARS, National Animal Disease CenterFollow
Victoria Mack, Washington State University, Pullman
Lindsay M. Fry, Washington State University, Pullman & USDA, ARS, Animal Disease Research Unit
William C. Davis, Washington State University, PullmanFollow

Date of this Version

2016

Citation

Park KT, ElNaggar MM, Abdellrazeq GS, Bannantine JP, Mack V, Fry LM, et al. (2016) Phenotype and Function of CD209+ Bovine Blood Dendritic Cells, Monocyte-Derived-Dendritic Cells and Monocyte-Derived Macrophages. PLoS ONE 11(10): e0165247. doi:10.1371/journal. pone.0165247

Comments

This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

Abstract

Phylogenic comparisons of the mononuclear phagocyte system (MPS) of humans and mice demonstrate phenotypic divergence of dendritic cell (DC) subsets that play similar roles in innate and adaptive immunity. Although differing in phenotype, DC can be classified into four groups according to ontogeny and function: conventional DC (cDC1 and cDC2), plasmacytoid DC (pDC), and monocyte derived DC (MoDC). DC of Artiodactyla (pigs and ruminants) can also be sub-classified using this system, allowing direct functional and phenotypic comparison of MoDC and other DC subsets trafficking in blood (bDC). Because of the high volume of blood collections required to study DC, cattle offer the best opportunity to further our understanding of bDC and MoDC function in an outbred large animal species. As reported here, phenotyping DC using a monoclonal antibody (mAb) to CD209 revealed CD209 is expressed on the major myeloid population of DC present in blood and MoDC, providing a phenotypic link between these two subsets. Additionally, the present study demonstrates that CD209 is also expressed on monocyte derived macrophages (MoΦ). Functional analysis revealed each of these populations can take up and process antigens (Ags), present them to CD4 and CD8 T cells, and elicit a T-cell recall response. Thus, bDC, MoDC, and MoΦ pulsed with pathogens or candidate vaccine antigens can be used to study factors that modulate DC-driven T-cell priming and differentiation ex vivo.