Intraspecies comparative genomics of three strains of Orientia tsutsugamushi with different antibiotic sensitivity

Authors

Hsiao-Mei Liao, Center for Biologics Evaluation and Research, Food and Drug Administration
Chien-Chung Chao, Naval Medical Research Center
Haiyan Lei, Center for Biologics Evaluation and Research, Food and Drug Administration
Bingjie Li, Center for Biologics Evaluation and Research, Food and Drug Administration
Shien Tsai, Center for Biologics Evaluation and Research, Food and Drug Administration
Guo-Chiuan Hung, Center for Biologics Evaluation and Research, Food and Drug Administration
Wei-Mei Ching, Naval Medical Research Center
Shyh-Ching Lo, Center for Biologics Evaluation and Research, Food and Drug Administration

Date of this Version

2017

Citation

Genomics Data 12 (2017) 84–88

Comments

U.S. Government Work

Abstract

We recently reported the genome of Orientia tsutsugamushi (OT) strain Karp (GenBank Accession #: NZ_LYMA00000000.2, https://www.ncbi.nlm.nih.gov/nuccore/NZ_LYMA00000000.2) with N2 Mb in size through clone-based sequencing and high throughput genomic shotgun sequencing (HTS). The genomes of OT strains AFSC4 and AFSC7 were similarly sequenced by HTS Since strains AFSC4 (GenBank Accession #: NZ_LYMT00000000.1, https://www.ncbi.nlm.nih.gov/nuccore/1035784408) and AFSC7 (GenBank Accession #: NZ_LYMB00000000.1, https://www.ncbi.nlm.nih.gov/nuccore/1035854767) were more resistant to antibiotics than strain Karp, we conducted comparative analysis of the three draft genomes annotated by RAST server aimed to identify possible genetic bases of difference in microbial antibiotic sensitivity. Intraspecies comparative genomics analysis of the three OT strains revealed that two ORFs encoding hypothetical proteins in both strains AFSC4 and AFSC7 are absent in strain Karp.