Formation of Influenza Virus Particles Lacking Hemagglutinin on the Viral Envelope

Authors

Asit K. Pattnaik, University of Nebraska-LincolnFollow
Donald J. Brown, University of California at Los Angeles
Debi P. Nayak, University of California at Los Angeles

Document Type

Article

Date of this Version

1986

Citation

JOURNAL OF VIROLOGY, Dec. 1986, p. 994-1001

Comments

Copyright © 1986, American Society for Microbiology

Abstract

We investigated the intraceUular block in the transport of hemagglutinin (HA) and the role of HA in virus particle formation by using temperature-sensitive (Is) mutants (1s134 and 1s61S) of inOuenza virus AlWSN/33. We found that at the nonpermissive temperature (39.5°C), the exit of ts HA from the rough endoplasmic reticulum to the Golgi complex was blocked and that no additional block was apparent in either the exit from the Golgi complex or post-Golgi complex transport. When MDBK ceUs were infected with these mutant viruses, they produced noninfectious virus particles at 39.5°C. The efficiency of particle formation at 39.5°C was essentiaUy the same for both wild-type (wt) and Is virus-infected cells. When compared with the wt virus produced at either 33 or 39.5°C or the ts virus formed at 33°C, these noninfectious virus particles were lighter in density and lacked spikes on the envelope. However, they contained the full complement of genomic RNA as well as aU of the structural polypeptides of inOuenza virus with the exception of HA. In these spikeless particles, HA could not be detected at the limit of 0.2% of the HA present in wt virions. In contrast, neuraminidase appeared to be present in a twofold excess over the amount present in Is virus formed at 33°C. These observations suggest that the presence of HA is not an obligatory requirement for the assembly and budding of inftuenza virus particles from infected ceUs. The implications of these results and the possible role of other viral proteins in inOuenza virus morphogenesis are discussed.