EHDV-2 Infection Prevalence Varies in Culicoides sonorensis after Feeding on Infected White-Tailed Deer over the Course of Viremia

Authors

Sandra Y. Mendiola, University of GeorgiaFollow
Mary K. Mills, 2 & University of South Carolina AikenFollow
Elin Maki, USDA, Agricultural Research ServiceFollow
Barbara S. Drolet, USDA, Agricultural Research ServiceFollow
William C. Wilson, USDA, Agricultural Research ServiceFollow
Roy D. Berghaus, University of GeorgiaFollow
David E. Stallknecht, University of GeorgiaFollow
Jonathan Breitenbach, USDA, Agricultural Research ServiceFollow
D. Scott McVey, USDA, Agricultural Research ServiceFollow
Mark G. Ruder, University of GeorgiaFollow

ORCID IDs

https://orcid.org/0000-0002-7414-4512

https://orcid.org/0000-0002-7877-9224

https://orcid.org/0000-0002-2161-6491

Document Type

Article

Date of this Version

2019

Citation

Viruses 2019, 11, 371; doi:10.3390/v11040371

Comments

2019 by the authors.

Abstract

Epizootic hemorrhagic disease viruses (EHDVs) are arboviral pathogens of white-tailed deer and other wild and domestic ruminants in North America. Transmitted by various species of Culicoides, EHDVs circulate wherever competent vectors and susceptible ruminant host populations co-exist. The impact of variation in the level and duration of EHDV viremia in white-tailed deer (Odocoileus virginianus) on Culicoides infection prevalence is not well characterized. Here we examined how infection prevalence in a confirmed North American vector of EHDV-2 (Culicoides sonorensis) varies in response to fluctuations in deer viremia. To accomplish this, five white-tailed deer were experimentally infected with EHDV-2 and colonized C. sonorensis were allowed to feed on deer at 3, 5, 7, 10, 12, 14, 18, and 24 days post infection (dpi). Viremia profiles in deer were determined by virus isolation and titration at the same time points. Blood-fed Culicoides were assayed for virus after a 10-day incubation (27 ◦C) period. We found that increases in deer EHDV blood titers significantly increased both the likelihood that midges would successfully acquire EHDV and the proportion of midges that reached the titer threshold for transmission competence. Unexpectedly, we identified four infected midge samples (three individuals and one pool) after feeding on one deer 18 and 24 dpi, when viremia was no longer detectable by virus isolation. The ability of ruminants with low-titer viremia to serve as a source of EHDV for blood-feeding Culicoides should be explored further to better understand its potential epidemiological significance.