Veterinary and Biomedical Sciences, Department of
Document Type
Article
Date of this Version
7-2017
Citation
Vaccine 35:5 (July 2017), pp. 782–788.
doi: 10.1016/j.vaccine.2016.12.040
Abstract
Because porcine reproductive and respiratory syndrome virus (PRRSV) exhibits extensive genetic variation among field isolates, characterizing the extent of cross reactivity of immune responses, and most importantly cell-mediated immunity (CMI), could help in the development of broadly cross-protective vaccines. We infected 12 PRRSV-naïve pigs with PRRSV strain FL12 and determined the number of interferon (IFN)-γ secreting cells (SC) by ELISpot assay using ten type 2 and one type 1 PRRSV isolates as recall antigens. The number of IFN-γ SC was extremely variable among animals, and with exceptions, late to appear. Cross reactivity of IFN-γ SC among type 2 isolates was broad, and we found no evidence of an association between increased genetic distance among isolates and the intensity of the CMI response. Comparable to IFN-γ SC, total antibodies evaluated by indirect immunofluorescence assay (IFA) were cross reactive; however, neutralizing antibody titers could be detected only against the strain used for infection. Finally, we observed a moderate association between homologous IFN-γ SC and neutralizing antibodies.
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Comments
Copyright © 2016 Elsevier Ltd. Used by permission.