Use of Affinity Chromatography in Developing Acridinium Ester-Labeled Antibodies for an Immunometric Assay of Parathyrin

Authors

• David S. Hage, University of Nebraska - LincolnFollow
• Bob Taylor, Mayo Clinic/Foundation, Rochester, MN
• Pat Schryver, Mayo Clinic/Foundation, Rochester, MN
• Pai C. Kao, Mayo Clinic/Foundation, Rochester, MN

Document Type

Article

Date of this Version

1991

Citation

CLINICAL CHEMISTRY, Vol. 37, No. 1, 1991, pp. 117-118.

Comments

Copyright (c) 1991 American Association for Clinical Chemistry.

Abstract

In developing an immunometric assay of intact parathyrin (parathyroid hormone, PTH), we found that affinity chromatography is a useful tool in purifying and optimizing the labeling conditions for acridinium ester-labeled antibodies. ... In summary, affinity chromatography was found to be useful in the purification of acridinium ester-labeled antibodies, particularly for removing denatured antibodies from the prepared label and for monitoring the amount of active labeled antibodies produced.