Department of Chemistry
Date of this Version
1992
Citation
CLINICAL CHEMISTRY, Vol.38, No.2, 1992, pp. 303-304.
Abstract
Reversed-phase high-performance liquid chromatography (RP-HPLC) is an important tool in the purification of radiolabeled peptides and proteins for immunoassay. However, for some proteins and peptides it is difficult to achieve reproducible behavior in RP-HPLC because of the low recovery of these compounds. Factors that can be varied to improve recovery include the strength or pH of the mobile phase, the chain length and spacing of groups on the reversed-phase support, and the flow rate or steepness of the elution gradient (1-5). ... In summary, we obtained better recovery and more reproducible chromatographic behavior for labeled 1-34 PTHrP with an albumin-pretreated reversed-phase column than with a new, untreated reversed-phase column. This treatment is simple and inexpensive and should be generally applicable for use in the purification of other radiolabeled peptides and proteins.
Included in
Analytical, Diagnostic and Therapeutic Techniques and Equipment Commons, Biochemistry, Biophysics, and Structural Biology Commons, Chemicals and Drugs Commons, Medicinal-Pharmaceutical Chemistry Commons
Comments
Copyright (c) 1992 American Association for Clinical Chemistry.