Published Research - Department of Chemistry

 

Date of this Version

1991

Citation

CLINICAL CHEMISTRY, Vol. 37, No. 1, 1991, pp. 117-118.

Comments

Copyright (c) 1991 American Association for Clinical Chemistry.

Abstract

In developing an immunometric assay of intact parathyrin (parathyroid hormone, PTH), we found that affinity chromatography is a useful tool in purifying and optimizing the labeling conditions for acridinium ester-labeled antibodies. ... In summary, affinity chromatography was found to be useful in the purification of acridinium ester-labeled antibodies, particularly for removing denatured antibodies from the prepared label and for monitoring the amount of active labeled antibodies produced.

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