Food Science and Technology Department


First Advisor

Dr. Bing Wang

Date of this Version



Avello, C. (2021). Risk-based Evaluation of Treatments for Water Used at a Pre-harvest Stage to Mitigate Microbial Contamination of Fresh Raspberry in Chile. Theses, Dissertations, and Student Research in Department of Food Science & Technology, University of Nebraska-Lincoln.


A THESIS Presented to the Faculty of The Graduate College at the University of Nebraska In Partial Fulfillment of Requirements For the Degree of Master of Science, Major: Food Science and Technology, Under the Supervision of Professor Dr. Bing Wang. Lincoln, Nebraska: May 2021

Copyright © 2021 Constanza Paz Avello Lefno


The global agricultural sectors are facing challenges of providing food for a rapidly growing population while still meeting appropriate food quality and safety standards. Chile, a South American country with great climatic and soil diversity, is positioned among the top ten agricultural exporters in the world.

Berries are considered highly prioritized produce in terms of microbiological hazards. The work presented in this thesis primarily focuses on the control of microbial contamination in water used at a pre-harvest stage, for enhanced quality and safety of raspberry products in Chile destined for export.

Two studies were conducted to achieve the goals. In the first study, a systematic review was carried out to characterize water treatments based on their efficacy of reducing E. coli contamination, and in-field feasibility. The second study employed a quantitative simulation model to analyze the impact of water quality on E. coli contamination on fresh raspberries at the arrival of importers’ border.

Compiling findings of the two studies, suggestions on water treatment suitable for raspberry farms were provided. Independent, science-based assessment was conducted and highlighted the most relevant aspects that will help the Chilean food safety authorities with tools to suggest solutions to raspberry producers.

Advisor: Bing Wang