Comparative Assessment of Human Exposure to Antibiotic-Resistant Salmonella due to the Consumption of Various Food Products in the United States

Authors

Yifan Wu, University of Nebraska-LincolnFollow

First Advisor

Bing Wang

Date of this Version

8-2022

Document Type

Article

Citation

Wu, Y. (2022). Comparative Assessment of Human Exposure to Antibiotic-Resistant Salmonella due to the Consumption of Various Food Products in the United States. (Master Thesis, University of Nebraska- Lincoln, Lincoln, NE, United States).

Comments

A THESIS Presented to the Faculty of The Graduate College at the University of Nebraska In Partial Fulfillment of Requirements For the Degree of Master of Science, Major: Food Science and Technology, Under the supervision of Professor Bing Wang. Lincoln, Nebraska: August 2022

Copyright © 2022 Yifan Wu

Abstract

Antibiotic resistance (AR) was increasingly recognized as a global and national problem. Prevention efforts are hampered by a lack of complete understanding of how transmission pathways contribute to human AR exposure. Many reports have indicated the presence of antibiotic-resistant bacteria in foods at retail, suggesting that food consumption, animal-derived foods in particular, can represent a significant source of AR exposure among consumers. The presence of Salmonella, including antibiotic-resistant Salmonella, has been frequently reported in terrestrial animal-derived foods such as meat, poultry, and dairy products, as well as in aquaculture products. Identification of the significant food sources that harbor relatively substantial antibiotic-resistant Salmonella is the key for the design and implementation of effective and target AR mitigation strategies. Thus, a systematic evaluation of the relative contribution of different food sources to human antibiotic-resistant Salmonella was imperative.

This thesis aimed to gather qualitative and quantitative information about the contamination of antibiotic-resistant non-typhoidal Salmonella in various retail foods in the U.S. and identify knowledge gaps using systematic review (SR) and meta-analysis (MA) approaches. The data on resistant Salmonella concentration in foods has not been found. Resistant Salmonella prevalence in regulated commodities (beef, chicken, turkey, pork) and other food categories were conducted for major antibiotic classes. Generally, poultry, pork, and turkey had a higher prevalence of resistant Salmonella than beef, while vegetables and imported foods (mainly spices in documented studies) had a lower prevalence. For antibiotics classes, tetracycline resistance was the most prevalent across major commodities. There is a moderate level of resistance to beta-lactam antibiotics, but the significance in clinical practice indicates a potential threats to public health.

Another objective was to develop a stochastic comparative exposure assessment model to estimate the relative contribution of various animal-derived food groups to overall foodborne exposure to cephem-resistant Salmonella. The model consists of four modules: retail, transport, storage, and preparation. Generally, the results showed that ground beef and chicken parts accounted for the largest proportion of total exposure to cephem-resistant Salmonella compared to pork cuts and ground turkey. The contamination level in products at retail and cooking temperature were the top influencing factors of the foodborne exposure for all food products evaluated in the present study.

Foodborne illness source attribution is the foundation of a risk-based food safety system. The present project provides a risk-based estimation of the degree to which different food categories are responsible for resistant Salmonella infections. With these estimates, target prevention measures can be designed and implemented to effectively mitigate the AR threat to public health attributable to the food consumption.

Advisor: Bing Wang