U.S. Department of Agriculture: Agricultural Research Service, Lincoln, Nebraska
Date of this Version
3-2017
Citation
ANNUAL REPORT OF THE BEAN IMPROVEMENT COOPERATIVE, No. 60, March 2017. Published by USDA.
Abstract
INTRODUCTION: The common bean is a traditional crop in México which is produced in many agricultural regions throughout the country. Nonetheless, its quality depends of environmental factors that have place during the plant development; in this sense, the metabolic response to these factors can be modify by abiotic factors such as a limited water supply, and this can be observed in different plant tissues. The objective was to obtain a secondary metabolites differentiation regarding the phenylpropanoid pathway of two Flor de Junio (FJ) bean cultivars.
MATERIAL AND METHODS: Cultivars FJ Dalia and Victoria were sown in July 2016 at the INIFAP-Zacatecas Research Center. Plants were subjected to different water supply regimens: T1, 100/50 % of water availability in vegetative/reproductive stage; T2, 75/12.5; T3, 50,100 and T4, 12.5,75. Sampling was conducted when plants reached the reproductive stage; foliar tissue was collected and immediately drown in liquid nitrogen, freeze dry and processed in a domestic grinder, samples were kept at room temperature until analysis. Extraction of polyphenols was performed using 1 g of sample and 10 mL of acidic acetone [acetone/water/acetic acid (70:29.5:0.5, v/v/v)] as described by Xu et al. (2007). Content of total phenols (mg GAE/g), total flavonoids (mg CAE/g), condensed tannins (mg CAE/g) and anthocyanins (mg C3GE/g) was determined.
Comments
U.S. government work.