U.S. Department of Agriculture: Agricultural Research Service, Lincoln, Nebraska
Document Type
Article
Date of this Version
3-2017
Citation
ANNUAL REPORT OF THE BEAN IMPROVEMENT COOPERATIVE, No. 60, March 2017. Published by USDA.
Abstract
INTRODUCTION: Common bean plants are often subjected to water deficiency due to the large extent of territory destined to production under rainfed conditions, which may lead to a critical abiotic stress that affects plant physiology and development. Plants that are exposed to drought stress generally produce higher levels of secondary metabolites, including triterpenoids such as saponins (Nasrollahi et al., 2014). The objective was to quantify the total saponins content of different plant tissue of two Flor de Junio (FJ) common bean cultivars.
MATERIAL AND METHODS: Cultivars FJ Dalia and Victoria were sown in July 2016 at the INIFAP-Zacatecas Research Center, under a rainout shelter to protect against rainfall; plants were subjected to different water supply regimens: 1, 50/50 % of water availability in vegetative/reproductive stage; 2, 100/100; 3, 50,100 and 4, 100,50. Root and foliar tissue were collected when plants reached the reproductive stage, samples were immediately freeze dry and processed in a domestic grinder. In brief, total saponins were extracted adding 4 mL of hexane to 0.5 g of dry sample, after shaking for 6 h, samples were centrifuged and acetonitrile was added to the hexanoic extracts (Hiai et al., 1976). Total saponins quantification was conducted on the basis of a reaction with vanillin and sulfuric acid. Results were expressed as μg equivalent of oleanolic acid/gram of dry sample (μg OAE/g).
Comments
U.S. government work.