Molecular mapping of greenbug resistance genes Gb2 and Gb6 in T1AL.1RS wheat-rye translocations

Authors

H. Lu, Texas AgriLife Research
Jackie C. Rudd, Texas AgriLife Research and Extension CenterFollow
J. D. Burd, USDA ARS
Y. Weng, USDA-ARSFollow

Document Type

Article

Date of this Version

2011

Comments

Published in Plant Breeding, 129, 472—476 (2010). doi:10.1111/j.1439-0523.2009.01722.x

Abstract

Two greenbug resistance genes Gb2 and Gb6 derived from the same donor rye line 'Insave', are present in wheat germplasm lines 'Amigo' and 'GRS1201', respectively as 1AL.1RS wheat-rye translocations. The allelic relationship between the two genes has not been determined, and no molecular markers for Gb6 are available. In this study, molecular mapping of Gb2 and Gb6 was performed in a mapping population derived from a cross between 'N96L9970' (Gb6Gb6) and 'TAM 107' (Gb2Gb2). Segregation among F_2:3 families of host responses to infestation of greenbug biotypes E and KS-1 revealed that Gb2 and Gb6 were different linked loci in 1RS. Gb2 and Gb6 were 15.8 cM apart with Gb6 being distal to Gb2. Despite the low number of marker polymorphisms between the parental lines, eight markers linked with Gb2 and Gb6 were identified. The closest marker, XIA294, was 11.4 cM proximal to Gb2. Deletion mapping indicated that both Gb2 and Gb6 were physically located in the satellite region of 1RS.