Veterinary and Biomedical Sciences, Department of

 

First Advisor

Greg Somerville

Second Advisor

Etsuko Moriyama

Date of this Version

Spring 4-27-2022

Document Type

Article

Comments

A THESIS Presented to the Faculty of The Graduate College at the University of Nebraska In Partial Fulfillment of Requirements For the Degree of Master of Science, Major: Veterinary Science, Under the Supervision of Professors Greg Somerville and Etsuko Moriyama. Lincoln, Nebraska: April, 2022

Copyright © 2022 Connor M. Aylor

Abstract

Clostridium perfringens is a common member of gut microbiota in healthy animals, but can also be an important pathogen in human and veterinary medicine. It produces several protein toxins that contribute to both histotoxic and enteric diseases in animals. Necrotic enteritis in poultry has been associated with the NetB toxin of C. perfringens; however, this toxin alone is insufficient to cause disease in infected chickens. While considerable research has focused on the presence of toxins and virulence factors, little has been done to assess the function of metabolic factors on the ability of the bacteria to cause disease. In this study, the metabolic differences are examined using genomic sequence analysis between genomes of stains of C. perfringens that are associated with necrotic enteritis. Several metabolic pathways are examined, which show different metabolic genes across C. perfringens lineages.

Advisors: Greg Somerville and Etsuko Moriyama

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