Veterinary and Biomedical Sciences, Department of
School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research
First Advisor
Hiep L. X. Vu
Second Advisor
John Dustin Loy
Third Advisor
Sarah Sillman
Date of this Version
4-2022
Document Type
Thesis
Citation
A thesis presented to the faculty of the Graduate College at the University of Nebraska in partial fulfillment of requirements for the degree of Master of Science
Major: Veterinary Sciences
Under the supervision of Professor Hiep L. X. Vu
Lincoln, Nebraska, April 2022
Abstract
Influenza A virus of the swine (IAV-S) is an economically important swine pathogen that has the potential to spread to humans, thus posing an ongoing public health concern due to its zoonotic potential. Hemagglutinin (HA), the most abundant viral envelope protein, is known to be the key protective antigen. Anti-HA antibodies alone, have been shown to prevent IAV infection. In this study we evaluated the feasibility of a recombinant tri segmented Pichinde virus (PICV) as a viral vector to deliver IAV-S hemagglutinin antigen in pigs. Four groups of weaned pigs (T01-04) were immunized twice with PBS, rPICV-GFP as a vector control, rPICV-H3 and H3-protein, respectively, at day 0 and 21 followed by an intra-tracheal challenge with a wild-type H3N2 IAV-S strain on day 42. T03 and T04 groups seroconverted and exhibited high titers of plasma neutralizing antibodies after vaccination. Post challenge infection, undetectable or minimal levels of influenza virus shedding, and lung lesions were observed in T03 and T04 groups whereas high levels of shedding and lung lesions were observed in T01 and T02 groups. Overall, both rPICV-H3 and H3-protein were able to confer solid protection in pigs and no significant difference in terms of protection was observed between these two vaccine types. These results provide the basis for the continuous development of the rPICV to use as a potential viral vector vaccine in pigs.
Advisor: Hiep L. X. Vu
Included in
Animal Sciences Commons, Immunology of Infectious Disease Commons, Infectious Disease Commons, Large or Food Animal and Equine Medicine Commons, Medicinal and Pharmaceutical Chemistry Commons, Medicinal Chemistry and Pharmaceutics Commons, Pharmaceutics and Drug Design Commons, Therapeutics Commons, Veterinary Infectious Diseases Commons, Virology Commons, Viruses Commons
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Copyright © 2022, Sushmita Kumari. Used by permission