Robert W. Hutkins, Professor
Food Science and Technology Department
Food Industry Complex
University of Nebraska-Lincoln
Lincoln, Nebraska 68583-0919 United States

The Hutkins Lab studies bacteria important in fermented foods and in human health. We are particularly interested in understanding the molecular basis for metabolism of prebiotic sugars by lactic acid bacteria and bifidobacteria (so-called probiotic bacteria). How these prebiotics shift the intestinal microbiota in humans and animals is also being studied. We are also interested in the anti-adhesive properties of oligosaccharides and the molecular mechanisms involved in pathogen binding to the surface of host cells.

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2011

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Specialized adaptation of a lactic acid bacterium to the milk environment: the comparative genomics of Streptococcus thermophilus LMD-9, Yong Jun Goh, Caitlin Goin, Sarah O'Flaherty, Eric Altermann, and Robert W. Hutkins

2007

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Functional analysis of the fructooligosaccharide utilization operon in Lactobacillus paracasei 1195, Yong Jun Goh, Jong-Hwa Lee, and Robert W. Hutkins

2006

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Identification of a Putative Operon Involved in Fructooligosaccharide Utilization by Lactobacillus paracasei, Yong Jun Goh, Andrew K. Benson, Vicki Schlegel, Jong-Hwa Lee, and Robert W. Hutkins

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Making Yogurt at Home, Robert W. Hutkins

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Prebiotic Galactooligosaccharides Reduce Adherence of Enteropathogenic Escherichia coli to Tissue Culture Cells, Kari Schoaf, George L. Mulvey, Glen D. Armstrong, and Robert W. Hutkins

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Prebiotic Galactooligosaccharides Reduce Adherence of Enteropathogenic Escherichia coli to Tissue Culture Cells, Kari Shoaf, George L. Mulvey, Glen D. Armstrong, and Robert W. Hutkins

2004

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Measurements of Fitness and Competition in Commensal Escherichia coli and E. coli O157:H7 Strains, Lisa M. Durso, David R. Smith, and Robert W. Hutkins

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Catabolite Repression and Virulence Gene Expression in Listeria monocytogenes, Stefanie Evans Gilbreth, Andrew K. Benson, and Robert W. Hutkins

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DNA Sequence Analysis of Regions Surrounding blaCMY-2 from Multiple Salmonella Plasmid Backbones, W.P. Giles, Andrew K. Benson, M.E. Olson, Robert W. Hutkins, J.M. Whichard, P.L. Winokur, and P.D. Fey

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Regulation of Transcription of Compatible Solute Transporters by the General Stress Sigma Factor, σB, in Listeria monocytogenes, Mehmet Sevket Cetin, Chaomei Zhang, Robert W. Hutkins, and Andrew K. Benson

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Regulation of Transcription of Compatible Solute Transporters by the General Stress Sigma Factor, σB, in Listeria monocytogenes, Mehmet Sevket Cetin, Chaomei Zhang, Robert W. Hutkins, and Andrew K. Benson

2003

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Metabolism of Fructooligosaccharides by Lactobacillus paracasei 1195, Handan Kaplan and Robert W. Hutkins

2002

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Displacement of Escherichia coli O157:H7 from Rumen Medium Containing Prebiotic Sugars, Albane de Vaux, Mark Morrison, and Robert W. Hutkins

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Memorial Khem Shahani, Robert W. Hutkins, Burt Maxcy, and Steve Taylor

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Discovering lactic acid bacteria by genomics, Todd Klaenhammer, Eric Altermann, Fabrizio Arigoni, Alexander Bolotin, Fred Breidt, Jeffrey Broadbent, Raul Cano, Stephane Chaillou, Josef Deutscher, Mike Gasson, Maarten van de Gutche, Jean Guzzo, Axel Hartke, Trevor Hawkins, Pascal Hols, Robert W. Hutkins, Michiel Kleerebezem, Jan Kok, Oscar Kuipers, Mark Lubbers, Emanuelle Maguin, Larry McKay, David Mills, Arjen Nauta, Ross Overbeek, Herman Pel, David Pridmore, Milton Saier, Douwe van Sinderen, Alexei Sorokin, James Steele, Daniel O'Sullivan, Willem de Vos, Bart Weimer, Monique Zagorec, and Roland Seizen

2000

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Role of σB in Adaptation of Listeria monocytogenes to Growth at Low Temperature, Lynne A. Becker, Stefanie N. Evans, Robert W. Hutkins, and Andrew K. Benson

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Fermentation of Fructooligosaccharides by Lactic Acid Bacteria and Bifidobacteria, Handan Kaplan and Robert W. Hutkins

1999

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Mutational Analysis of the Role of HPr in Listeria monocytogenes, Douglas P. Christensen, Andrew K. Benson, and Robert W. Hutkins

1998

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Identification of the Gene Encoding the Alternative Sigma Factor σB from Listeria monocytogenes and Its Role in Osmotolerance, Lynne A. Becker, Mehmet Sevket Cetin, Robert W. Hutkins, and Andrew K. Benson

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Cloning and Expression of the Listeria monocytogenes Scott A ptsH and ptsI Genes, Coding for HPr and Enzyme I, Respectively, of the Phosphotransferase System, Douglas P. Christensen, Andrew K. Benson, and Robert W. Hutkins

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United States Patent: SILICALITE MEMBRANE AND METHOD FOR THE SELECTIVE RECOVERY AND CONCENTRATION OF ACETONE AND BUTANOL FROM MODEL ABE SOLUTIONS AND FERMENTATION BROTH, Michael M. Meagher, Nasibuddin Qureshi, and Robert W. Hutkins

1997

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Listeria monocytogenes Scott A Transports Glucose by High-Affinity and Low-Affinity Glucose Transport Systems, Corinne Parker and Robert W. Hutkins

1995

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Cloning and Characterization of the Galactokinase Gene from Streptococcus thermophilus, Azlin Mustapha, Robert W. Hutkins, and Gerald W. Hirnstein

1994

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Glucose Uptake by Listeria monocytogenes Scott A and Inhibition by Pediocin JD, Douglas P. Christensen and Robert W. Hutkins

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Browning of Mozzarella Cheese During High Temperature Pizza Baking, Beatriz Matzdorf, Susan L. Cuppett, Laurie J. Keeler, and Robert W. Hutkins

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Isolation of Galactose-Fermenting Thermophilic Cultures and Their Use in the Manufacture of Low Browning Mozzarella Cheese, Kamla K. Mukhurjee and Robert W. Hutkins

1993

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United States Patent: COMPOSITION AND METHOD FOR INHIBITING PATHOGENS AND SPOILAGE ORGANISMS IN FOOD, Robert W. Hutkins, Elaine D. Berry, and Michael B. Liewen

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pH Homeostasis in Lactic Acid Bacteria, Robert W. Hutkins and Nancy L. Nannen

1992

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Collapse of the Proton Motive Force in Listeria monocytogenes Caused by a Bacteriocin Produced by Pediococcus acidilactici, Douglas P. Christensen and Robert W. Hutkins

1991

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Lactose Uptake Driven by Galactose Efflux in Streptococcus thermophilus: Evidence for a Galactose-Lactose Antiporter, Robert W. Hutkins and Carina Ponne

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Intracellular pH Effects in Lactic Acid Bacteria, Nancy L. Nannen and Robert W. Hutkins

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Proton-Translocating Adenosine Triphosphatase Activity in Lactic Acid Bacteria, Nancy L. Nannen and Robert W. Hutkins

1987

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Betaine Transport Imparts Osmotolerance on a Strain of Lactobacillus acidophilus, Robert W. Hutkins, William L. Ellefson, and Eva R. Kashket

1986

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Use of Galactose-Fermenting Streptococcus thermophilus in the Manufacture of Swiss, Mozzarella, and Short-Method Cheddar Cheese, Robert W. Hutkins, S.M. Halambeck, and H.A. Morris

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Phosphotransferase Activity in Clostridium acetobutylicum from Acidogenic and Solventogenic Phases of Growth, Robert W. Hutkins and Eva R. Kashket

1985

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Galactokinase Activity in Streptococcus thermophilus, Robert W. Hutkins, Howard A. Morris, and Larry L. McKay

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Galactose Transport in Streptococcus thermophilus, Robert W. Hutkins, Howard A. Morris, and Larry L. McKay

1979

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G74-449 Making Yogurt at Home, Robert W. Hutkins and Susan Sumner