James Van Etten
William Allington Distinguished Professor of Plant Pathology
Professor, School of Biological Sciences
Nebraska Center for Virology

Lab webpage: http://ncv.unl.edu/vanettenlab/

Dr. Van Etten has studied a family of large, polyhedral, plaque forming, dsDNA viruses that replicate in certain eukaryotic unicellular Chlorella-like green algae. One surprising feature of these viruses is that at least some of them encode for DNA modification and restriction systems. In fact the virus infected algae are a new source of type II DNA restriction endonucleases and the first source from a nonprokaryotic system. The plaque assay, the ability to synchronously infect the host, the short life cycle, and the ability of the viruses to undergo homologous recombination make them excellent model systems for studying DNA replication and gene expression in a photosynthetic eukaryote.

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2007

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Structure and Function of a Chlorella Virus Encoded Glycosyltransferase, Ying Zhang, Ye Xiang, James L. Van Etten, and Michael G. Rossmann

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Chloroviruses Encode a Bifunctional dCMP-dCTP Deaminase That Produces Two Key Intermediates in dTTP Formation, Yuanzheng Zhang, Frank Maley, Gladys F. Maley, Garry Duncan, David Dunigan, and James L. Van Etten

2006

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Virion-Associated Restriction Endonucleases of Chloroviruses, Irina V. Agarkova, David Dunigan, and James L. Van Etten

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Cloning of Nt.CviQII Nicking Endonuclease and Its Cognate Methyltransferase: M.CviQII Methylates AG Sequence, Siu-Hong Chan, Zhenyu Zhu, David Dunigan, James L. Van Etten, and Shuang-yong Xu

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Phycodnaviruses: A peek at genetic diversity, David Dunigan, Lisa A. Fitzgerald, and James L. Van Etten

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Potassium Ion Channels of Chlorella Viruses Cause Rapid Depolarization of Host Cells during Infection, Florian Frohns, Anja Kasmann, Detlef Kramer, Britta Schafer, Mario Mehmel, Ming Kang, James L. Van Etten, Sabrina Gazzarrini, Ana Morino, and Gerhard Thiel

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Chlorella virus MT325 encodes water and potassium channels that interact synergistically, Sabrina Gazzarrini, Ming Kang, Svetlana Epimashko, James L. Van Etten, Jack Dainty, Gerhard Thiel, and Anna Moroni

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Chlorella Viruses, Takashi Yamada, Hideki Onimatsu, and James L. Van Etten

2005

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Chlorovirus: A Genus of Phycodnaviridae that Infects Certain Chlorella-Like Green Algae, Ming Kang, David Dunigan, and James L. Van Etten

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Functional Implication of the tRNA Genes Encoded in the Chlorella Virus PBCV-l Genome, Da Young Lee, Michael V. Graves, James L. Van Etten, and Tae-Jin Choi

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The Structure of a T=169d Algal Virus, PBCV-1, at 15Å Resolution, X. Yan, V. Bowman, N. H. Olson, James Gurnon, James L. Van Etten, Michael G. Rossmann, and T. S. Baker

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Chlorella Virus-Encoded Deoxyuridine Triphosphatases Exhibit Different Temperature Optima, Yuanzheng Zhang, Hideaki Moriyama, Kohei Homma, and James L. Van Etten

2004

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Biography of James L. Van Etten, Christen Brownlee and James L. Van Etten

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Small potassium ion channel proteins encoded by chlorella viruses, Ming Kang, Anna Moroni, Sabrina Gazzarrini, Dario DiFrancesco, Gerhard Thiel, Maria Severino, and James L. Van Etten

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Glycosyltransferases encoded by viruses, Nicolas Markine-Goriaynoff, Laurent Gillet, James L. Van Etten, Haralambos Korres, Naresh Verma, and Alain Vanderplasschen

2003

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Are Chlorella Viruses a Rich Source of Ion Channel Genes?, Ming Kang, Anna Moroni, Sabrina Gazzarrini, and James L. Van Etten

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Unusual Life Style of Giant Chlorella Viruses, James L. Van Etten

2002

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Isolation and characterization of a virus that infects Emiliania huxleyi (Haptophyta), Tonje Castberg, Runar Thyrhaug, Aud Larsen, Ruth-Anne Sandaa, Mikal Heldal, James L. Van Etten, and Gunnar Bratbak

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Ornithine Decarboxylase Encoded by Chlorella Virus PBCV-1, Tiara A. Morehead, James R. Gurnon, Byron Adams, Kenneth Nickerson, Lisa A. Fitzgerald, and James L. Van Etten

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The Structure and Evolution of the Major Capsid Protein of a Large, Lipid-Containing DNA Virus, Narayanasamy Nandhagopal, Alan Simpson, James Gurnon, Xiadong Yan, Timothy Baker, Michael V. Graves, James L. Van Etten, and Michael Rossman

2000

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A Potassium Channel Protein Encoded by Chlorella Virus PBCV-1, B. Plugge, S. Gazzarrini, M. Nelson, R. Cerana, James L. Van Etten, C. Derst, D. DiFrancesco, A. Moroni, and G. Thiel

1999

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Chlorella Virus PBCV-1 Encodes a Functional Homospermidine Synthase, Annette Kaiser, Michael Vollmert, Dorothea Tholl, Michael V. Graves, James Gurnon, Weimei Xing, Amber D. Lisec, Kenneth Nickerson, and James L. Van Etten

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CAN LARGE dsDNA-CONTAINING VIRUSES PROVIDE INFORMATION ABOUT THE MINIMAL GENOME SIZE REQUIRED TO SUPPORT LIFE?, James L. Van Etten

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Giant Viruses Infecting Algae, James L. Van Etten and Russel Meints

1997

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Hyaluronan Synthase of Chlorella Virus PBCV-1, Paul L. DeAngelis, Wei Jing, Michael V. Graves, Dwight E. Burbank, and James L. Van Etten

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Chlorella Virus PBCV-1 Encodes a Homolog of the Bacteriophage T4 UV Damage Repair Gene denV, Masakazu Furuta, John Schrader, Holly Schrader, Tyler Kokjohn, Simon Nyaga, Amanda McCullough, R. Stephen Lloyd, Dwight Burbank, Dorit Landstein, Leslie C. Lane, and James L. Van Etten

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Characterization of an ATP-Dependent DNA Ligase Encoded by Chlorella Virus PBCV-1, C. Kiong Ho, James L. Van Etten, and Stewart Shuman

1996

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Expression and Characterization of an RNA Capping Enzyme Encoded by Chlorella Virus PBCV-1, C. Kiong Ho, James L. Van Etten, and Stewart Shuman

1993

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Sequencing two DNA templates in five channels by digital compression, Michaela Nelson, Yanping Zhang, David L. Steffens, Reingard Grabherr, and James L. Van Etten

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Leptospira Genomes Are Modified at 5'-GTAC, David Ralph, Quideng Que, James L. Van Etten, and Michael McClelland

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Evidence for Virus-Encoded Glycosylation Specificity, Ing-Nang Wang, Yu Li, Quideng Que, Meenakshi Bhattacharya, Leslie C. Lane, William G. Chaney, and James L. Van Etten

1991

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Viruses and Virus-like Particles of Eukaryotic Algae, James L. Van Etten, Leslie C. Lane, and Russel H. Meints

1988

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Chlorella Viruses Isolated in China, Yanping Zhang, Dwight Burbank, and James L. Van Etten

1986

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Restriction Endonuclease Activity Induced by PBCV-1 Virus Infection of a Chlorella-Like Green Alga, Yuannan Xia, Dwight Burbank, Lothar Uher, Dietrmar Rabussay, and James L. Van Etten

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DNA Methyltransferase Induced by PBCV-1 Virus Infection of a Chlorella-Like Green Alga, Yuannan Xia and James L. Van Etten

1985

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Synthesis and Localization of a Development-Specific Protein in Sclerotia of Sclerotinia sclerotiorum, Geraldine Russo and James L. Van Etten

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A Survey for Viruses from Fresh Water That Infect a Eucaryotic Chlorella-Like Green Alga, James L. Van Etten, Cecil H. Van Etten, Jeffrey Johnson, and Dwight Burbank

1983

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Biosynthesis and Degradation of Storage Protein in Spores of the Fungus Botryodiplodia theobromae, Gary Petersen, Kurt Dahlberg, and James L. Van Etten

1982

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Viruses of symbiotic Chlorella-like algae isolated from Paramecium bursaria and Hydra viridis, James L. Van Etten, Russel H. Meints, Daniel Kuczmarski, Dwight E. Burbank, and Kit Lee

1980

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In Vitro Translation of the Three Bacteriophage φ6 RNAs, Diane A. Cuppels, James L. Van Etten, Dwight E. Burbank, Leslie C. Lane, and Anne K. Vidaver

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Semiconservative Synthesis of Single-Stranded RNA by Bacteriophage φ6 RNA Polymerase, James L. Van Etten, Dwight E. Burbank, Diane A. Cuppels, Leslie C. Lane, and Anne K. Vidaver

1979

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Presence of a Major (Storage?) Protein in Dormant Spores of the Fungus Botryodiplodia theobromae, James L. Van Etten, S. N. Freer, and B. K. McCune

1978

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Simple Procedure for Disruption of Fungal Spores, James L. Van Etten and Shelby Freer

1977

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Absence of Spermine in Filamentous Fungi, Kenneth W. Nickerson, Larry D. Dunkle, and James L. Van Etten

1976

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Comparative Properties of Bacteriophage φ6 and φ6 Nucleocapsid, James L. Van Etten, Les Lane, Carlos Gonzalez, James Partridge, and Anne Vidaver

1975

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Intermediates in the Biosynthesis of Double-Stranded Ribonucleic Acids of Bacteriophage φ6, D. L. Coplin, James L. Van Etten, James A. Koski, and A. K. Vidaver

1974

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Influence of Molecular Weights of Bacteriophage φ6 Double-Stranded Ribonucleic Acids on Interferon Induction, W.J. Kleinschmidt, James L. Van Etten, and A. K. Vidaver

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Physiological and Morphological Correlation of Rhizopus stolonifer Spore Germination, James L. Van Etten, Lee Bulla Jr., and Grant St. Julian

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Base Composition and Hybridization Studies of the Three Double-Stranded RNA Segments of Bacteriophage φ6, James L. Van Etten, A. K. Vidaver, R. K. Koski, and J. P. Burnett

1973

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Characteristics of Deoxyribonucleic Acid Polymerase Isolated from Spores of Rhizopus stolonifer , Cheng-Shung Gong, Larry Dunkle, and James L. Van Etten

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RNA Polymerase Activity Associated with Bacteriophage φ6, James L. Van Etten, A. K. Vidaver, R. K. Koski, and J. S. Semancik

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Bacteriophage φ6: a Lipid-Containing Virus of Pseudomonas phaseolicola, Anne K. Vidaver, R. K. Koski, and James L. Van Etten

1972

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Protein Synthesis During Fungal Spore Germination: Differential Protein Synthesis During Germination of Botryodiplodia theobromae Spores, James L. Van Etten, Henry Roker, and Eric Davies

1971

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Preparation of Biologically Active Ribosomal Subunits from Fungal Spores, James L. Van Etten

1969

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Protein Synthesis During Fungal Spore Germination. IV. Transfer Ribonucleic Acid from Germinated and Ungerminated Spores, James L. Van Etten, R. Kent Koski, and Mahmoud El-Olemy

1968

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Protein Synthesis During Fungal Spore Germination II. Aminoacyl-soluble Ribonucleic Acid Synthetase Activities During Germination of Botryodiplodia theobromae Spores, James L. Van Etten and Robert Brambl

1966

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Changes in Fungi with Age I. Chemical Composition of Rhizoctonia solani and Sclerotium bataticola , David Gottlieb and James L. Van Etten

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Changes in Fungi with Age II. Respiration and Respiratory Enzymes of Rhizoctonia solani and Sclerotium bataticola, James L. Van Etten, H. Peter Molitoris, and David Gottlieb

1965

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Biochemical Changes During the Growth of Fungi: II. Ergosterol and Fatty Acids in Penicillium atrovenetum, David Gottlieb and James L. Van Etten

1964

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Biochemical Changes During the Growth of Fungi: I. Nitrogen Compounds and Carbohydrate Changes in Penicillium atrovenetum, David Gottlieb and James L. Van Etten