Scientists at the Roman L. Hruska U.S. Meat Animal Research Center (USMARC) develop new technology in order to increase the efficiency of livestock production and benefit consumers. The USMARC was authorized by Congress on June 16, 1964, and development began in the spring of 1966 on 35,000 acres near Clay Center, Nebraska. Presently, research programs are using a female breeding population of 6,500 cattle of 18 breeds, 4,000 sheep of 10 breeds, and 700 swine litters per year. The USMARC is administered by the Agricultural Research Service (ARS) within the United States Department of Agriculture (USDA). On October 10, 1978, President Carter signed into law a bill which renamed the research facility after former Senator Roman L. Hruska of Nebraska.

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2014

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Characterization of Escherichia coli O157:H7 Strains from Contaminated Raw Beef Trim during “High Event Periods”, Terrance M. Arthur, James L. Bono, and Norasak Kalchayanand

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The impact of cow nutrient status during the second and third trimesters on age at puberty, antral follicle count, and fertility of daughters, R. A. Cushman, Anthony K. McNeel, and Harvey C. Freetly

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Effects of decreased dietary roughage concentration on energy metabolism and nutrient balance in finishing beef cattle, K. E. Hales, T. M. Brown-Brandl, and H. C. Freetly

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Dystrophin insufficiency causes selective muscle histopathology and loss of dystrophin-glycoprotein complex assembly in pig skeletal muscle, Katrin Hollinger, Cai X. Yang, Robyn E. Montz, Dan Nonneman, Jason W. Ross, and Joshua T. Selsby

2013

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Characterization of Escherichia coli O157:H7 Strains Isolated from Supershedding Cattle, Terrance M. Arthur, Rafiq Ahmed, Margo Chase-Topping, Norasak Kalchayanand, John W. Schmidt, and James L. Bono

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Characterization of E. coli O157:H7 Strains Resulting from Contamination of Raw Beef Trim during High Event Periods, Terrance M. Arthur, James L. Bono, and Norasak Kalchayanand

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Characterization of Ovine Nectin-4, a Novel Peste des Petits Ruminants Virus Receptor, Jamie Birch, Nicholas Juleff, Michael P. Heaton, Ted Kalbfleisch, James Kijas, and Dalan Bailey

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Analysis of feeding behavior of group housed growing–finishing pigs, T.M. Brown-Brandl, G.A. Rohrer, and R.A. Eigenberg

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Development and characterization of two porcine monocyte-derived macrophage cell lines, Carol G. Chitko-McKown, Stephen K. Chapes, Laura C. Miller, Penny K. Riggs, M. Teresa Ortega, Benedict T. Green, and Richard D. McKown

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PHYSIOLOGY AND ENDOCRINOLOGY SYMPOSIUM: The current status of heat shock in early embryonic survival and reproductive effi ciency, R. A. Cushman

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Identification of an ionotropic glutamate receptor AMPA1/GRIA1 polymorphism in crossbred beef cows differing in fertility, R. A. Cushman, J. R. Miles, L. A. Rempel, T. G. McDaneld, L. A. Kuehn, C. G. Chitko-McKown, D. Nonneman, and S. E. Echternkamp

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Effect of postweaning diet on ovarian development and fertility in replacement beef heifers, D. R. Eborn, R. A. Cushman, and S. E. Echternkamp

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Substantial within-Animal Diversity of Salmonella Isolates from Lymph Nodes, Feces, and Hides of Cattle at Slaughter, Sara E. Gragg, Guy H. Loneragan, Kendra K. Nightingale, Dayna M. Brichta-Harhay, Henry Ruiz, Jacob R. Elder, Lyda G. Garcia, Markus F. Miller, Alejandro Echeverry, Rosa G. Ramírez Porras, and Mindy M. Brashears

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Lineage and Genogroup-Defining Single Nucleotide Polymorphisms of Escherichia coli O157:H7, Woo Kyung Jung, James L. Bono, Michael L. Clawson, Shana R. Leopold, Smriti Shringi, and Thomas E. Besser

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Alternative Models in Genetic Analyses of Carcass Traits Measured by Ultrasonography in Guzerá cattle: A Bayesian Approach, Jairo Azevedo Junior, José C. Souza, R. A. Cushman, Marco C. A. M. Bink, Caio A. Perazza, Sarah L. Meirelles, and Tarcisio M. Gonçalves

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Reducing Assembly Complexity of Microbial Genomes with Single-Molecule Sequencing, Sergey Koren, Gregory P. Harhay, Timothy P. L. Smith, James L. Bono, Dayna M. Harhay, Scott D. Mcvey, Diana Radune, Nicholas H. Bergman, and Adam M. Phillipy

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Effects of TMEM154 haplotypes 1 and 3 on susceptibility to ovine progressive pneumonia virus following natural exposure in sheep, K. A. Leymaster, C. G. Chitko-McKown, M. L. Clawson, G. P. Harhay, and M. P. Heaton

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Adipose and Muscle Tissue Gene Expression of Two Genes (NCAPG and LCORL) Located in a Chromosomal Region Associated with Cattle Feed Intake and Gain, Amanda K. Lindholm-Perry, Larry Kuehn, William T. Oliver, Andrea K. Sexten, Jeremy R. Miles, Lea Ann Rempel, R. A. Cushman, and Harvey C. Freetly

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The Plasminogen Activator System in the Ovine Placentome During Late Gestation and Stage-Two of Parturition, Anthony K. McNeel, Robert A. Cushman, and Jeffrey L. Vallet

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Technical Report: Serial collections of placentomes during parturition in cattle and subsequent reproductive performance, Anthony K. McNeel, Jeff D. Ondrak, and R. A. Cushman

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A simulation-based approach for evaluating and comparing the environmental footprints of beef production systems, C. A. Rotz, B. J. Isenberg, K. R. Stackhouse-Lawson, and E. J. Pollak

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Small Ruminant Lentivirus Genetic Subgroups Associate with Sheep TMEM154 Genotypes, Lucia H. Sider, Michael P. Heaton, Carol G. Chitko-McKown, Greg P. Harhay, Timothy P. L. Smith, Kreg A. Leymaster, William W. Laegreid, and Michael L. Clawson

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BREEDING AND GENETICS SYMPOSIUM: Networks and pathways to guide genomic selection, W. M. Snelling, R. A. Cushman, J. W. Keele, C. Maltecca, M. G. Thomas, M. R. S. Fortes, and A. Reverter

2012

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Phylogeny of Shiga Toxin-Producing Escherichia coli O157 Isolated from Cattle and Clinically Ill Humans, James L. Bono, Timothy P. L. Smith, James E. Keen, Gregory P. Harhay, Tara G. McDaneld, Robert E. Mandrell, Woo Kyung Jung, Thomas E. Besser, Peter Gerner-Smidt, Martina Bielaszewska, Helge Karch, and Michael L. Clawson

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Evidence That the Autoimmune Regulator Gene Influences Thymic Production of Ovarian Antigens and Prevents Autoimmune-Mediated Premature Reproductive Senescence, R. A. Cushman

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Using Resistivity Arrays to Monitor Groundwater Impacts near Runoff Holding Ponds, Roger A. Eigenberg and Bryan L. Woodbury

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PHYSIOLOGY AND ENDOCRINOLOGY SYMPOSIUM: Factors controlling puberty in beef heifers, P. M. Fricke and R. A. Cushman

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Reduced Lentivirus Susceptibility in Sheep with TMEM154 Mutations, Michael P. Heaton, Michael L. Clawson, Carol G. Chitko-Mckown, Kreg A. Leymaster, Timothy P. L. Smith, Gregory P. Harhay, Stephen N. White, Lynn M. Herrmann-Hoesing, Michelle R. Mousel, Gregory S. Lewis, Theodore S. Kalbfleisch, James E. Keen, and William W. Laegreid

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Evaluation of bovine chemerin (RARRES2) gene variation on beef cattle production traits, Amanda K. Lindholm-Perry, Larry A. Kuehn, Lea A. Rempel, Timothy P.L. Smith, R. A. Cushman, Tara G. McDaneld, Tommy L. Wheeler, Steven D. Shackelford, David A. King, and Harvey C. Freetly

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Contributions of the maternal uterine environment and piglet genotype on weaning survivability potential: I. Development of neonatal piglets after reciprocal embryo transfers between Meishan and White crossbred gilts, Jeremy R. Miles, J. L. Vallet, J. J. Ford, Brad A. Freking, R. A. Cushman, William T. Oliver, and Lea Ann Rempel

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MicroRNA expression profile in bovine cumulus–oocyte complexes: Possible role of let-7 and miR-106a in the development of bovine oocytes, J.R. Miles, T.G. McDaneld, R.T. Wiedmann, R.A. Cushman, S.E. Echternkamp, J.L. Vallet, and T.P.L. Smith

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Consistent divergence times and allele sharing measured from cross-species application of SNP chips developed for three domestic species, J. M. Miller, J. W. Kijas, M. P. Heaton, J. C. Mcewan, and D. W. Coltman

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A Defect in Dystrophin Causes a Novel Porcine Stress Syndrome, Dan Nonneman, Tami Brown-Brandl, Shuna A. Jones, Ralph T. Wiedmann, and Gary A. Rohrer

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Responses to Varroa destructor and Nosema ceranae by several commercial strains of Australian and North American honeybees (Hymenoptera: Apidae), Thomas E. Rinderer, Benjamin P. Oldroyd, Amanda M. Frake, Lilia I. de Guzman, and Lelania Bourgeois

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PHYSIOLOGY AND ENDOCRINOLOGY SYMPOSIUM: How single nucleotide polymorphism chips will advance our knowledge of factors controlling puberty and aid in selecting replacement beef females, W. M. Snelling, Robert Cushman, M. R. S. Fortes, A. Reverter, G. L. Bennett, J. W. Keele, L. A. Kuehn, T. G. McDaneld, Mark Thallman, and M. G. Thomas

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Dual – Species Biofilm Formation by Shiga – toxin Producing Escherichia coli O157:H7 and O26:H11 serotypes, Rong Wang, Norasak Kalchayanand, John W. Schmidt, and Joseph M. Bosilevac

2011

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Assessment of pubertal development of boars derived from ultrasonographic determination of testicular diameter, J.J. Ford and T.H. Wise

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Determinants of bovine thermal response to heat and solar radiation exposures in a field environment, Brad Scharf, Michael J. Leonard, Robert L. Weaber, Terry L. Mader, G. Leroy Hahn, and Donald E. Spiers

2010

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Super Shedding of Escherichia coli O157:H7 by Cattle and the Impact on Beef Carcass Contamination, Terrance M. Arthur, Dayna M. Brichta-Harhay, Joseph M. Bosilevac, Norasak Kalchayanand, Steven D. Shackelford, Tommy L. Wheeler, and Mohammad Koohmaraie

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Tenderness – An Enzymatic View, Caroline M. Kemp, Paul L. Sensky, Ronald G. Bardsley, Peter J. Buttery, and Tim Parr

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REVIEW: Preweaning, Postweaning, and Carcass Trait Comparisons for Progeny Sired by Subtropically Adapted Beef Sire Breeds at Various US Locations, F. A. Thrift, J. O. Sanders, M. A. Brown, A. H. Brown Jr, A. D. Herring, D. G. Riley, S. M. DeRouen, J. W. Holloway, W. E. Wyatt, R. C. Vann, C. C. Chase Jr., D. E. Franke, L. V. Cundiff, and J. F. Baker

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Proportion of the Litter Farrowed, Litter Size, and Progesterone and Estradiol Effects on Piglet Birth Intervals and Stillbirths, J. L. Vallet, J. R. Miles, T. M. Brown-Brandl, and J. A. Nienaber

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Repellency of a Wax-Based Catnip-Oil Formulation against Stable Flies, Junwei J. Zhu, Christopher A. Dunlap, Robert W. Behle, Dennis R. Berkebile, and Brian Wienhold

2008

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Enumeration of Salmonella from Poultry Carcass Rinses via Direct Plating Methods, D. M. Brichta-Harhay, T. M. Arthur, and M. Koohmaraie

2007

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Enumeration of Salmonella and Escherichia coli O157:H7 in Ground Beef, Cattle Carcass, Hide and Faecal Samples Using Direct Plating Methods, D. M. Brichta-Harhay, T. M. Arthur, J. M. Bosilevac, M. N. Guerini, N. Kalchayanand, and M. Koohmaraie

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Comparative Response of Rams and Bulls to Long-Term Treatment with Gonadotropin-Releasing Hormone Analogs, H. Jiménez-Severiano, M. J. D'Occhio, D. D. Lunstra, M. L. Mussard, T. L. Davis, W. J. Enright, and J. E. Kinder

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Placental Expression of the Membrane Form of Folate Binding Protein During Pregnancy in Swine, J. G. Kim and J. L. Vallet

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Interventions to Reduce/Eliminate Escherichia coli O157:H7 in Ground Beef, M. Koohmaraie, T. M. Arthur, J. M. Bosilevac, D. M. Brichta-Harhay, N. Kalchayanand, S. D. Shackelford, and T. L. Wheeler

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Computing numerator relationships between any pair of animals, L. Dale Van Vleck

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Comparison of Meat Composition from Offspring of Cloned and Conventionally Produced Boars, S. C. Walker, R. K. Christenson, R. P. Ruiz, D. E. Reeves, S. L. Pratt, F. Arenivas, N. E. Williams, B. L. Bruner, and I. A. Polejaeva

2006

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Quantification of Calpastatin Using an Optical Surface Plasmon Resonance Biosensor, G. H. Geesink, J. G. P. van der Palen, M. P. Kent, E. Veiseth, G. Hemke, and M. Koohmaraie

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Lack of Effect of Metyrapone and Exogenous Cortisol on Early Porcine Conceptus Development, H. G. Klemcke, J. L. Vallet, and R. K. Christenson

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Contribution of Postmortem Muscle Biochemistry to the Delivery of Consistent Meat Quality with Particular Focus on the Calpain System, M. Koohmaraie and G. H. Geesink

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Effects of a Single Foot Rot Incident on Weight Performance of Feedlot Steers, G. K. TIBBETTS, T. M. DEVIN, D. GRIFFIN, J. E. KEEN, and G. P. RUPP

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Germplasm Evaluation Program Progress Report No. 23, T. L. Wheeler, Larry V. Cundiff, L. Dale Van Vleck, G.D. Snowder, R.M. Thallman, Steven D. Shackelford, and M. Koohmaraie

2005

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Genetic Relationships Between Male and Female Reproductive Traits in Beef Cattle, G. Gargantini, L. V. Cundiff, D. D. Lunstra, and L. D. Van Vleck

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Perspectives for artificial insemination and genomics to improve global swine populations, Roger J. Gerrits, Joan K. Lunney, Lawrence A. Johnson, Vernon G. Pursel, Robert R. Kraeling, Gary A. Rohrer, and John R. Dobrinsky

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Post-Harvest Interventions to Reduce/Eliminate Pathogens in Beef, M. Koohmaraie, T. M. Arthur, J. M. Bosilevac, M. Guerini, S. D. Shackelford, and T. L. Wheeler

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On-line Classification of US Select Beef Carcasses for Longissimus Tenderness Using Visible and Near-Infrared Reflectance Spectroscopy, S. D. Shackelford, T. L. Wheeler, and M. Koohmaraie

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Allelic Variation in the Erythropoietin Receptor Gene is Associated with Uterine Capacity and Litter Size in Swine, J. L. Vallet, B. A. Freaking, K. A. Leymaster, and R. K. Christenson

2004

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Germplasm Evaluation Program Progress Report No. 22, Larry V. Cundiff, T. L. Wheeler, Keith Gregory, Steven D. Shackelford, M. Koohmaraie, R.M. Thallman, G.D. Snowder, and L. Dale Van Vleck

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Breed Comparisons of Weight, Weight Adjusted for Condition Score, Height, and Condition Score of Beef Cows, Department of Animal Science, University of Nebraska, Lincoln; L. V. Cundiff; and L. D. Van Vleck

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Comparison of Longhorn- and Red Poll-Sired Calves from Crossbred Dams Calving at Two or Three Years of Age, X H. Liu, A Perez Marquez, L. V. Cundiff, and L. D. Van Vleck

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Apoptosis and porcine reproductive and respiratory syndrome virus, Laura C. Miller and James M. Fox

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Development of Optimal Protocol for Visible and Near-Infrared Reflectance Spectroscopic Evaluation of Meat Quality, S. D. Shackelford, T. L. Wheeler, and M. Koohmaraie

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Factors Regulating Lamb Longissimus Tenderness are Affected by Age at Slaughter, E. Veiseth, S. D. Shackelford, T. L. Wheeler, and M. Koohmaraie

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Soil quality: a review of the science and experiences in the USA, B. J. Wienhold, S. S. Andrews, and D. L. Karlen

2003

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Molecular Cloning and Endometrial Expression of Porcine Amphiregulin, Jong G. Kim, Jeffrey L. Vallet, and Ronald K. Christenson

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Frequency of Luteinizing Hormone Pulses in Cattle Influences Duration of Persistence of Dominant Ovarian Follicles, Follicular Fluid Concentrations of Steroids, and Activity of Insulin-Like Growth Factor Binding Proteins, F. N. Kojima, E. G. M. Bergfeld, M. E. Wehrman, A. S. Cupp, K. E. Fike, D. V. Mariscal-Aguayo, T. Sanchez-Torres, M. Garcia-Winder, D. T. Clopton, A. J. Roberts, and J. E. Kinder

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Germ Cell Development in Meishan and White Composite Gilts, Susan A. McCoard, Thomas H. Wise, and J. Joe Ford

2002

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Characterization of Porcine Uterine Estrogen Sulfotransferase, J. G. Kim, J. L. Vallet, G. A. Rohrer, and R. K. Christenson

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Regulation of Endometrial Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) in the Ewe, W. J. McGuire, K. Imakawa, K. Tamura, C. S. R. Meka, and R. K. Christenson

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Genetic Parameters of Reproductive Traits in Sheep, A. Rosati, E. Mousa, L. D. Van Vleck, and L. D. Young

2001

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Germplasm Evaluation Program- Progress Report No. 21, Larry V. Cundiff, T. L. Wheeler, Keith Gregory, Steven D. Shackelford, M. Koohmaraie, R.M. Thallman, and L. Dale Van Vleck

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Germplasm Evaluation Program- Progress Report No. 20, Larry V. Cundiff, T. L. Wheeler, Steven D. Shackelford, M. Koohmaraie, R.M. Thallman, Keith Gregory, and L. Dale Van Vleck

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Use of Heart Girth to Predict Body Weight of Working Oxen in the Ethiopian Highlands, M. R. Goe, J. R. Alldredge, and D. Light

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Characterization of Uterine Epidermal Growth Factor During Early Pregnancy in Pigs, J. G. Kim, J. L. Vallet, and R. K. Christenson

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The Role of Cholecystokinin in the Expression of Seasonal Variation in the Feed Intake and Eating Pattern of Red Deer (Cervus elaphus), S. M. Rhind, S. R. McMillen, J. C. Pekas, and E. Duff

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Structure of the Genes for Porcine Endometrial Secreted and Membrane Folate Binding Proteins, J. L. Vallet, T. P. L. Smith, T. S. Sonstegard, M. Heaton, and S. C. Fahrenkrug

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Effect of Extraction Buffer on Estimating Calpain and Calpastatin Activity in Postmortem Ovine Muscle, E. Veiseth and M. Koohmaraie

2000

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Development and Demonstration of RNA Isolation and RT−PCR Procedures to Detect Escherichia coli O157:H7 Gene Expression on Beef Carcass Surfaces, E. D. Berry

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Germplasm Evaluation Program Progress Report No. 19, Larry V. Cundiff, Keith Gregory, T. L. Wheeler, Steven D. Shackelford, M. Koohmaraie, Harvey C. Freetly, and D.D. Lunstra

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Luteinizing Hormone Secretion as Affected by Hypophyseal Stalk Transection and Estradiol-17β in Ovariectomized Gilts, J. J. Ford, J. G. Berardinelli, R. K. Christenson, and L. L. Anderson

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Dehydrogenase and Oxoreductase Activities of Porcine Placental 11β-Hydroxysteroid Dehydrogenase, Harold G. Klemcke

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Identification and Characterization of a New Allele for the Beta Subunit of Follicle-Stimulating Hormone in Chinese Pig Breeds, M. D. Li, G. A. Rohrer, T. H. Wise, and J. J. Ford

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Porcine IgA allotypes are not equally transcribed or expressed in heterozygous swine, P. Navarro, R.K. Christensen, P. Weber, M. Rothschild, G. Ekhardt, and J.E. Butler

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Porcine Erythropoietin Receptor: Molecular Cloning and Expression in Embryonic and Fetal Liver, P. L. Pearson, T. P. L. Smith, T. S. Sonstegard, H. G. Klemcke, R. K. Christenson, and J. L. Vallet

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Mapping of Seven Bovine Cytokine Genes Involved in T-lymphocyte Growth, Differentiation and Immune Response, T. S. Sonstegard, W. M. Garrett, G. L. Bennett, S. M. Kappes, D. S. Zarlenga, and L. C. Gasbarre

1999

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Germplasm Evaluation Program- Progress Report No. 18, Larry V. Cundiff, Keith Gregory, T. L. Wheeler, Steven D. Shackelford, M. Koohmaraie, Harvey C. Freetly, and D.D. Lunstra

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A Single Nucleotide Polymorphism in the Bovine Kit Oncogene (Hardy−Zuckerman 4 Feline Sarcoma Viral (v-kit) Oncogene Homolog), M. D. Grosz and R. T. Stone

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Hydrogen-Deuterium Exchange and Ultraviolet Resonance Raman Spectroscopy of Bacteria in a Complex Food Matrix, Gregory P. Harhay and Gregory R. Siragusa

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Mapping of the SDHA Locus to Bovine Chromosome 20, T. S. Sonstegard and S. M. Kappes

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Mapping of the ucp1 locus to bovine chromosome 17, T. S. Sonstegard and S. M. Kappes

1998

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Germplasm Evaluation Program- Progress Report No. 17, Larry V. Cundiff, Keith Gregory, T. L. Wheeler, Steven D. Shackelford, M. Koohmaraie, Harvey C. Freetly, and D.D. Lunstra

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Comparative gene mapping: cytogenetic localization of PROC, EN1, ALPI, TNP1, and IL1B in cattle and sheep reveals a conserved rearrangement relative to the human genome, N. L. Lòpez-Corrales, T. S. Sonstegard, and T. P. L. Smith

1997

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Germplasm Evaluation Program- Progress ReportNo. 16, Larry V. Cundiff, Keith Gregory, T. L. Wheeler, Steven D. Shackelford, M. Koohmaraie, Harvey C. Freetly, and D.D. Lunstra

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Calculation Of Fractal Dimension From Cattle Thermoregulatory Responses, R. L. KORTHALS, Y. R. CHEN, G. L. HAHN, and R. A. EIGENBERG

1996

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A Search for Quantitative Trait Loci for Ovulation Rate in Cattle, A. N. Blattman, B. W. Kirkpatrick, and K. E. Gregory

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Germplasm Evaluation Program- Progress Report No. 15, Larry V. Cundiff, Keith E. Gregory, T. L. Wheeler, Steven D. Shackelford, M. Koohmaraie, Harvey C. Freetly, and D.D. Lunstra

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Use of a Newly Developed Rapid Microbial ATP Bioluminescence Assay to Detect Microbial Contamination on Poultry Carcasses, G. R. Siragusa, W. J. Dorsa, C. N. Cutter, L. J. Perino, and M. Koohmaraie